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Using in vitro liver models, we showed that these thiomethyl metabolites are directly linked to the elimination of the hepatotoxic NAPQI metabolites since S-CH 3 -APAP-S and SO-CH 3 -APAP-S are formed at high levels after exposure to NAQPI-derived metabolites (glutathione, cysteine, and mercapturate conjugates) in both primary human hepatocytes and HRG cells

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This selectivity represents a paradigm shift in antioxidant therapy: Hydroxyl radical specificity : Molecular hydrogen primarily neutralizes hydroxyl radicals (OH), considered the most cytotoxic reactive oxygen species with no known endogenous detoxification system, while leaving beneficial oxidative molecules intact